Mouse anti double-stranded RNA (J2)

Nordic-MUbio

Mouse anti double-stranded RNA (J2)

7

Mouse monoclonal antibody J2 can be used for ELISA, dsRNA-immunoblotting, immunoaffinity chromatography and in certain systems also for immunohistochemistry (see references). The optimum working dilution of the antibody for any specific application should be established by titration. Please note that nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gel electrophoresis, because the sensitivity of detection is considerably lower after blotting from agarose gels. Not for use for clinical purposes. For in vitro use only.

Dot Blot, Flow Cytometry, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Purification/Extraction/Isolation

dsRNA-immunoblotting;Dot blot;Flow Cytometry;ELISA;Immuno-affinity-chromatography;Immunocytochemistry;Immunohistochemistry

Primary antibodies

Research Use Only

Monoclonal

J2

1.00 mg/ml

Mouse

IgG2a

Mouse anti dsRNA

Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freunds adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to ...

-20°C or -80°C,2°C to 8°C

12352203