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ProductsBack/Mouse anti double-stranded RNA (J5)

Mouse anti double-stranded RNA (J5)

Research Use Only
10040500
Nordic-MUbio
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
Price on request
500 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti double-stranded RNA (J5)
  • Delivery Days Customer
    7
  • Application Supplier Note
    Mouse monoclonal antibody J5 can be used for ELISA, dsRNA-immunoblotting, immunoaffinity chromatography and in certain systems also for immunohistochemistry (see references). The optimum working dilution of the antibody for any specific application should be established by titration Please note that nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gel electrophoresis, because the sensitivity of detection is considerably lower after blotting from agarose gels. Not for use for clinical purposes. For in vitro use only.
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ELISA;Immunoblotting;Immunocytochemistry;Immunohistochemistry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    J5
  • Concentration
    1.00 mg/ml
  • Host
    Mouse
  • Isotype
    IgG2b
  • Scientific Description
    Mouse anti dsRNA
  • Source
    Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freunds adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to ...
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • 1) Schönborn, J., Oberstrass, J., Breyel, E., Tittgen, J., Schumacher, J. and Lukacs, N. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res.19, 2993-3000. 2) Lukacs, N. (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods 47, 255-272. 3) Lukacs, N. (1997) Detection of sense:antisense duplexes by structurespecific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford. 4) S. J. Richardson, A. Willcox, D. A. Hilton, S. Tauriainen, H. Hyoty, A. J. Bone, A. K. Foulis, N. G. Morgan. Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. J Clin Virol. (2010) 49(3); 180-5. doi: 10.1016/j.jcv.2010.07.015. 5) Levicán-Asenjo J, Soto-Rifo R, Aguayo F, Gaggero A, Leon O. Salmon cells SHK-1 internalize infectious pancreatic necrosis virus by macropinocytosis. J Fish Dis. 2019 Jul;42(7):1035-1046. doi: 10.1111/jfd.13009.