Mouse anti double-stranded RNA (K1)
10020200
ApplicationsOther Application, Flow Cytometry, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
Overview
- SupplierNordic-MUbio
- Product NameMouse anti double-stranded RNA (K1)
- Delivery Days Customer7
- ApplicationsOther Application, Flow Cytometry, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- Applications SupplierdsRNA-immunoblotting;ELISA;Immunoblotting;Immuno-affinity-chromatography;Immunocytochemistry;Immunohistochemistry;Flow Cytometry
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- Clone IDK1
- ConcentrationConcentration after reconstitution: 1.00 mg/ml as determined by A280 nm (A280 nm = 1.47 corresponds to 1 mg/ml antibody).
- Estimated PurityGel electrophoretically pure IgG antibody.
- HostMouse
- IsotypeIgG2a
- Scientific DescriptionMouse anti dsRNA
- SourceFemale DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate t
- Storage InstructionAfter reconstitution antibodies should be aliquoted and stored at -20 °C or -70 °C. After adding 10 mM sodium azide undiluted antibody can also be stored at +4 °C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided. When kept lyophilized the product will remain stable for 10 years at -20 °C or -70°C.
- UNSPSC12352203
References
- 1) Schönborn, J., Oberstrass, J., Breyel, E., Tittgen, J., Schumacher, J. and Lukacs, N. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res.19, 2993-3000. 2) Lukacs, N. (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods 47, 255-272. 3) Lukacs, N. (1997) Detection of sense:antisense duplexes by structurespecific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford. 4) S. J. Richardson, A. Willcox, D. A. Hilton, S. Tauriainen, H. Hyoty, A. J. Bone, A. K. Foulis, N. G. Morgan. Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. J Clin Virol. (2010) 49(3); 180-5. doi: 10.1016/j.jcv.2010.07.015.