Mouse anti double-stranded RNA (K2)
10030005
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
Overview
- SupplierNordic-MUbio
- Product NameMouse anti double-stranded RNA (K2)
- Delivery Days Customer7
- ApplicationsImmunoFluorescence, ELISA
- Applications SupplierELISA;Immunofluorescence
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- Clone IDK2
- ConcentrationUndiluted hybridoma supernatant
- HostMouse
- IsotypeIgM
- Scientific DescriptionMouse anti dsRNA
- SourceFemale DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate t
- Storage InstructionAfter delivery antibodies should be aliquoted and stored at -20 ° or -70 °C. After adding 10 mM sodium azide undiluted antibody can also be stored at +4 °C for a short period of time. For long term storage the antibody should be kept frozen. Repeated freezing/thawing cycles should be avoided.
- UNSPSC12352203
References
- 1) Schönborn, J., Oberstrass, J., Breyel, E., Tittgen, J., Schumacher, J. and Lukacs, N. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res.19, 2993-3000. 2) Lukacs, N. (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods 47, 255-272. 3) Lukacs, N. (1997) Detection of sense:antisense duplexes by structurespecific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford. 4) S. J. Richardson, A. Willcox, D. A. Hilton, S. Tauriainen, H. Hyoty, A. J. Bone, A. K. Foulis, N. G. Morgan. Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. J Clin Virol. (2010) 49(3); 180-5. doi: 10.1016/j.jcv.2010.07.015.