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ProductsBack/Mouse anti double-stranded RNA (K2)

Mouse anti double-stranded RNA (K2)

Research Use Only
10030010
Nordic-MUbio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
€ 855,00
10 ml
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti double-stranded RNA (K2)
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, ELISA
  • Applications Supplier
    Sandwich ELISA;dsRNA-immunoblotting;ELISA;Immunofluorescence
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clone ID
    K2
  • Concentration
    Undiluted hybridoma supernatant
  • Host
    Mouse
  • Isotype
    IgM
  • Scientific Description
    Mouse anti dsRNA
  • Source
    Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate t
  • Storage Instruction
    After delivery antibodies should be aliquoted and stored at -20 ° or -70 °C. After adding 10 mM sodium azide undiluted antibody can also be stored at +4 °C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided.
  • UNSPSC
    12352203

References

  • 1) Schönborn, J., Oberstrass, J., Breyel, E., Tittgen, J., Schumacher, J. and Lukacs, N. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res.19, 2993-3000. 2) Lukacs, N. (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods 47, 255-272. 3) Lukacs, N. (1997) Detection of sense:antisense duplexes by structurespecific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford. 4) S. J. Richardson, A. Willcox, D. A. Hilton, S. Tauriainen, H. Hyoty, A. J. Bone, A. K. Foulis, N. G. Morgan. Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. J Clin Virol. (2010) 49(3); 180-5. doi: 10.1016/j.jcv.2010.07.015.