Mouse anti E-Cadherin / Cadherin-1

Nordic-MUbio

Mouse anti E-Cadherin / Cadherin-1

7

MB2 recognizes both the 120 kD E-cadherin and its 80 kD trypsin-resistant extracellular part. MB2 is a functional antibody in that it inhibits cell-cell adhesion.

MB2 is useful for flow cytometry, immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent and 1:100 - 1:1000 for immunoblotting applications.

Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen

Flow Cytometry;Immunocytochemistry;Immunohistochemistry (frozen);Western Blotting

Primary antibodies

Research Use Only

Monoclonal

MB2

CDH1

cadherin 1

Arc-1; BCDS1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDHE; cell-CAM 120/80; ECAD; E-cadherin 1; epididymis secretory sperm binding protein; epithelial cadherin; LCAM; UVO; uvomorulin

Mouse

IgG2b

Cadherin-1

MB2 is a Mouse monoclonal IgG2b antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with MCF-7/AZ cells expressing E-cadherin at their cell surface.

Human

Human

12352203