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ProductsBack/Mouse anti E-Cadherin / Cadherin-1

Mouse anti E-Cadherin / Cadherin-1

MUB0300P
Nordic-MUbio
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetCDH1
0.1 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti E-Cadherin / Cadherin-1
  • Delivery Days Customer
    7
  • Application Supplier Note
    MB2 is useful for flow cytometry, immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent and 1:100 - 1:1000 for immunoblotting applications.
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    Flow Cytometry;Immunocytochemistry;Immunohistochemistry (frozen);Western Blotting
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MB2
  • Gene ID999
  • Target name
    CDH1
  • Target description
    cadherin 1
  • Target synonyms
    Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO, cadherin-1, CAM 120/80, E-cadherin 1, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), calcium-dependent adhesion protein, epithelial, cell-CAM 120/80, epididymis secretory sperm binding protein, epithelial cadherin, uvomorulin
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP12830
  • Protein Name
    Cadherin-1
  • Source
    MB2 is a Mouse monoclonal IgG2b antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with MCF-7/AZ cells expressing E-cadherin at their cell surface.
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of E Cadherin using anti-E Cadherin antibody (PB9561). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E Cadherin antigen affinity purified polyclonal antibody (Catalog # PB9561) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E Cadherin at approximately 130 kDa. The expected band size for E Cadherin is at 97 kDa.
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