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Mouse anti Human Caspase-8

X1175M
Nordic-MUbio
ApplicationsWestern Blot
Product group Antibodies
TargetCASP8
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human Caspase-8
  • Delivery Days Customer
    7
  • Application Supplier Note
    Detects proenzyme form of human Caspase-8 by Western blot analysis as well as one of the cleavage products. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot
  • Applications Supplier
    Western Blotting;Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    FLICE 1 1-37
  • Conjugate
    Unconjugated
  • Gene ID841
  • Target name
    CASP8
  • Target description
    caspase 8
  • Target synonyms
    ALPS2B; apoptotic cysteine protease; apoptotic protease Mch-5; CAP4; Casp-8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; caspase-8; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MCH5; MORT1-associated ced-3 homolog
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDQ14790
  • Protein Name
    Caspase-8
  • Scientific Description
    Cysteine-requiring Aspartate Protease-8; Apoptotic protease Mch-5; ICE-like apoptotic protease 5; FADD-like ICE
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-8 protein and mouse myeloma cells.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caspase 8 using anti-Caspase 8 antibody (A00042-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 8 antigen affinity purified polyclonal antibody (Catalog # A00042-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 8 at approximately 55KD. The expected band size for Caspase 8 is at 55KD.
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