Mouse anti Human CD103

Nordic-MUbio

Mouse anti Human CD103

7

PBMC: Add 10 microl of MAB/106 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2° to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20° C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.

Flow Cytometry

Flow Cytometry

Primary antibodies

Research Use Only

Monoclonal

AX.14

Unconjugated

ITGAE

integrin subunit alpha E

CD103, HUMINAE, integrin alpha-E, HML-1 antigen, antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide, human mucosal lymphocyte antigen 1, alpha polypeptide, integrin alpha-IEL, integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide), mucosal lymphocyte 1 antigen

Mouse

IgG1

Integrin alpha-E

CD103

See expiration date on vial

Human

12352203