Mouse anti Human CD19

Nordic-MUbio

Mouse anti Human CD19

7

PBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8 C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20 C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: Excites at 488nm and emits at 670nm. Store protected from light.

Flow Cytometry

Flow Cytometry

Primary antibodies

Research Use Only

Monoclonal

1G9

Unconjugated

CD19

CD19 molecule

B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CVID3; differentiation antigen CD19; T-cell surface antigen Leu-12

Mouse

IgG1

B-lymphocyte antigen CD19

CD19

See expiration date on vial

Human

12352203