Mouse anti Human CD2

Nordic-MUbio

Mouse anti Human CD2

7

Flow cytometry: PBMC: Add 10 microl of antibody to 1 million PBMCs in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8oC. Wash twice with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 microl of antibody to 100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for immunofluorescence analysis with a flow cytometer or microscope.

Flow Cytometry

Flow Cytometry

Primary antibodies

Research Use Only

Monoclonal

T6.3

Unconjugated

CD2

CD2 molecule

CD2 antigen (p50), sheep red blood cell receptor; erythrocyte receptor; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5

Mouse

IgG2a

T-cell surface antigen CD2

T-cell surface antigen CD2; CD2 cytoplasmic domain-binding protein

See expiration date on vial

Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by a mixed lymphocyte culture.

Human

12352203