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ProductsBack/Mouse anti Human CD25, conjugated with FITC

Mouse anti Human CD25, conjugated with FITC

Research Use Only
0252
Nordic-MUbio
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetIL2RA
Price on request
100 tests
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human CD25, conjugated with FITC
  • Delivery Days Customer
    7
  • Application Supplier Note
    PBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow Cytometry
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ITYV
  • Conjugate
    FITC
  • Gene ID3559
  • Target name
    IL2RA
  • Target description
    interleukin 2 receptor subunit alpha
  • Target synonyms
    CD25; IDDM10; IL-2 receptor subunit alpha; IL2R; IL-2R subunit alpha; IMD41; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP01589
  • Protein Name
    Interleukin-2 receptor subunit alpha
  • Scientific Description
    CD25*FITC
  • Shelf life instruction
    See expiration date on vial
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    CD25=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with PHA-activated human T-lymphocytes.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • 1. A Monoclonal Antibody (Anti-Tac) Reactive with Activated and Functionally Mature Human T Cells. Uchiyama T., Broder S., Waldmann TA, 1981 J. Immunol.126,1393. 2. Direct Demonstration of the Identity of T Cell Growth Factor Binding Protein and the Tac Antigen. Robb RJ, Greene WC, J. Exp. Med. 1983,158;1332. 3. Patients with HIV infection have a reduced proportion of lymphocytes expressing the IL2 receptor p55 chain (TAC, CD25). Zola H., Koh L.Y., Mantzioris B.X., Rhodes D., Clin. Immunol. Immunopathol. 1991 Ap:59(1):16-25. 4. Spontaneous lymphocyte proliferation in HTLV-I/II infection reflects preferential activation of CD8 and CD16/56 cell subsets. Prince H.E., Weber D.M., Jensen E.R., Clin. Immunol. Immunopathol. 1991, May;58(3):419-30. 5. Defective clonogenic potential of CD8+ T lymphocytes in patients with AIDS. Expansion in vivo of a nonclonogenic CD3+ CD8+ DR+ CD25- T cell population. Pantaleo G., Keonig S., Baseler M., Lane H.C., Fauci A.S., J. Immunol. 1990 Mar ; 144(5):1696-704.