Mouse anti Human CD4 FITC - CD28 PE

Nordic-MUbio

Mouse anti Human CD4 FITC - CD28 PE

7

PBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Flow Cytometry

Flow Cytometry

Research Use Only

FITC, RPE

CD4

CD4 molecule

CD4mut, IMD79, Leu-3, OKT4D, T4, T-cell surface glycoprotein CD4, CD4 antigen (p55), CD4 receptor, T-cell surface antigen T4/Leu-3

Mouse

IgG1

T-cell surface glycoprotein CD4

CD4 FITC - CD28 PE

See expiration date on vial

Human

CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.CD28=Derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice immunized with the HPB-ALL T-cell line.

2°C to 8°C

12352203