Mouse anti Human CD71, conjugated with FITC
0712
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetTFRC
Overview
- SupplierNordic-MUbio
- Product NameMouse anti Human CD71, conjugated with FITC
- Delivery Days Customer7
- Application Supplier NotePBMC: Add10 microl of MAB/10^6 PBMC in 100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 microl of MAB/100 microl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. Storage: Antibodies are supplied in PBS, 0.08% sodium azide and 0.2% protein carrier for FITC. Antibodies should be stored at 4-8oC. Monoclonal antibodies should not be frozen. Reagents are stable for the period shown on the vial label when stored properly.
- ApplicationsFlow Cytometry
- Applications SupplierFlow Cytometry
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDBGX.24
- ConjugateFITC
- Gene ID7037
- Target nameTFRC
- Target descriptiontransferrin receptor
- Target synonymsCD71; IMD46; p90; T9; TFR; TFR1; TR; transferrin receptor protein 1; TRFR
- HostMouse
- IsotypeIgG1
- Protein IDP02786
- Protein NameTransferrin receptor protein 1
- Scientific DescriptionCD71 FITC
- Shelf life instructionSee expiration date on vial
- ReactivityHuman
- Reactivity SupplierHuman
- Reactivity Supplier NoteDerived from the hybridization of mouse myeloma cells with spleen cells from BALB/c mice immunized with blast cells from pokeweed mitogen-stimulated peripheral blood cells.
- Storage Instruction2°C to 8°C
- UNSPSC12352203
References
- 1. Newman R, Schneider C, Sutherland R, Vodinelich L, Greaves M. The transferrin receptor. Trends Biochem Sci. 1982;1:397. 2. Schwarting R, Stein H. Cluster report: CD71. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press;1989:455-460. 3. Jefferies WA, Brandon MR, Hunt SV, Williams AF, Gatter KC, Mason DY. Transferrin receptor on endothelium of brain capillaries. Nature. 1984;312:162-163. 4. Judd W, Poodry CA, Strominger JL. Novel surface antigens expressed on dividing cells but absent from non-dividing cells. J Exp Med. 1980;152:1430. 5. Phillips JH, Le AM. Lanier LL. Natural killer cells activated in a human mixed lymphocyte response culture identified by expression of Leu-11 and class II histocompatibility antigens. J Exp Med. 1984;159:993-1008. 6. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. I. Normal erythroid development. Blood. 1987;69:255-263.