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ProductsBack/Mouse anti Human Cytochrome P450 2A6

Mouse anti Human Cytochrome P450 2A6

X2045M
Nordic-MUbio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCYP1A2
200 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human Cytochrome P450 2A6
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody can be used for Western blotting (1-2 microg/ml), immunofluorescence and immunohistochemistry (formalin-fixed, paraffin-embedded tissues). ELISA titer to be determined by end-user. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Western Blotting;ELISA;Immunohistochemistry;Immunohistochemistry (frozen & paraffin)
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    F16 P2 D8
  • Conjugate
    Unconjugated
  • Gene ID1544
  • Target name
    CYP1A2
  • Target description
    cytochrome P450 family 1 subfamily A member 2
  • Target synonyms
    aryl hydrocarbon hydroxylase; cholesterol 25-hydroxylase; CP12; CYPIA2; cytochrome P(3)450; cytochrome P450 1A2; cytochrome P450 4; cytochrome P450, family 1, subfamily A, polypeptide 2; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; cytochrome P450-P3; dioxin-inducible P3-450; flavoprotein-linked monooxygenase; hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; P3-450; P450 form 4; P450(PA); xenobiotic monooxygenase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05177
  • Protein Name
    Cytochrome P450 1A2
  • Scientific Description
    CYP2A6; CYP450 2A6 (EC 1.14.14.1), CYPIIA6, Coumarin 7-hydroxylase
  • Shelf life instruction
    See expiration date on vial
  • Source
    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with Synthetic peptide conjugated to ovalbumin derived from the C-terminus of CYP2A6 and mouse myeloma Ag8563 cells. Antigen identical in CYP2A6, 2A7 and 2A13.
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CYP1A2 using anti-CYP1A2 antibody (PB9545). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1A2 antigen affinity purified polyclonal antibody (Catalog # PB9545) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP1A2 at approximately 58 kDa. The expected band size for CYP1A2 is at 58 kDa.
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