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Anti-FBN2 Antibody

101-10510
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFBN2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Mouse Anti-Human FBN2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2201
  • Target name
    FBN2
  • Target description
    fibrillin 2
  • Target synonyms
    CCA; DA9; EOMD; fibrillin 5; fibrillin-2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    Human FBN2 monoclonal antibody (100 ug)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Immunofluorescence staining of U251 cells with CSB-PA008457LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of Fibrillin 2/FBN2 using anti-Fibrillin 2/FBN2 antibody (A03408-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibrillin 2/FBN2 antigen affinity purified polyclonal antibody (Catalog # A03408-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibrillin 2/FBN2 at approximately 315,250 kDa. The expected band size for Fibrillin 2/FBN2 is at 315 kDa.
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