Mouse anti Human HLA Class I Heavy Chain (Restricted expression)

Nordic-MUbio

Mouse anti Human HLA Class I Heavy Chain (Restricted expression)

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The mouse monoclonal antibody HCA2 recognize HLA class I heavy chains. The reactivity spectrum of HCA2 is composed of all HLA-A chains (except HLA-A24), as well as some HLA-B, HLA-C, HLA-E, HLA-F, and HLA-G chains.

The antibody HCA2 reacts preferentially with HLA-A locus heavy chains. HCA2 was raised against free class I heavy chains of HLA, to obtain antibodies that would still react with denatured class I antigens, as they occur in Western blotting, conventional light microscopical analysis of formalin-fixed, paraffin-embedded sections, and cryo-immuno-electron microscopy. HCA2 indeed retains strong reactivity with free class I heavy chains in Western blots. HCA2 in particular reacts in a locus-specific manner by biochemical criteria. Conditions are described for use of HCA2 in immunohistochemical staining of formalin-fixed, paraffin-embedded sections (see references). HCA2 also produces strong reactivity in immuno-electron microscopy. Its use allows the determination of tissue and subcellular distribution of class I antigens. Optimal antibody dilutions for the different applications should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

ELISA;Flow Cytometry;Immunohistochemistry (frozen & paraffin);Immunocytochemistry;Immunoprecipitation;Western Blotting;Immuno-cryo-electron microscopy;Immunohistochemistry (frozen & paraffin);ELISA;Immuno-cryo-electron microscopy

Primary antibodies

HCA2

HCA2 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with HLA-B7 and -B40 heavy chains.

Human

12352203