Bio-Connect

Mouse anti Human HLA Class I Heavy Chain (Restricted expression)

Research Use Only
MUB2036P
Nordic-MUbio
ApplicationsElectron Microscopy, Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
€ 226,00
0.1 mg
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human HLA Class I Heavy Chain (Restricted expression)
  • Delivery Days Customer
    7
  • Applications
    Electron Microscopy, Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ELISA;Flow Cytometry;Immunohistochemistry (frozen & paraffin);Immunocytochemistry;Immunoprecipitation;Western Blotting;Immuno-cryo-electron microscopy;Immunohistochemistry (frozen & paraffin);ELISA;Immuno-cryo-electron microscopy
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clone ID
    HCA2
  • Host
    Mouse
  • Isotype
    IgG1
  • Source
    HCA2 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with HLA-B7 and -B40 heavy chains.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight ...
  • UNSPSC
    12352203

References

  • 1. Stam NJ, Vroom TM, Peters PJ, Pastoors EB, Ploegh HL. HLA-A- and HLA-B-specific monoclonal antibodies reactive with free heavy chains in western blots, in formalin-fixed, paraffin-embedded tissue sections and in cryo-immuno-electron microscopy. Int. Immunol. 1990;2:113-125. 2. de Kruijf EM, van Nes JGH, Sajet A, Tummers QRJG, Putter H, Osanto S, Speetjens FM, Smit VTHBM, Liefers GJ, van de Velde CJH, Kuppen PJK. The predictive value of HLA Class I tumor cell expression and presence of intratumoral Tregs for chemotherapy in patients with early breast cancer. Clin Cancer Res 2010;16:1272-1280. 3. Hutter H, Hammer A, Blaschitz A, Hartmann M, Ebbesen P, Dohr G, Ziegler A, Uchanska-Ziegler B. Expression of HLA class I molecules in human first trimester and term placenta trophoblast. Cell Tissue Res 1996; 286:439–447. 4. Seitz C, Uchanska-Ziegler B, Zank A, Ziegler A. The monoclonal antibody HCA2 recognises a broadly shared epitope on selected classical as well as several non-classical HLA class I molecules. Mol Immunol. 1998;35:819-27. 5. Goodridge JP, Burian A, Lee N, Geraghty DE. HLA-F and MHC class I open conformers are ligands for NK cell Ig-like receptors. J Immunol. 2013;191(7):3553-62