Mouse anti Human HLA Class I Heavy Chain (Restricted expression)
MUB2037P
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman
Overview
- SupplierNordic-MUbio
- Product NameMouse anti Human HLA Class I Heavy Chain (Restricted expression)
- Delivery Days Customer7
- Antibody SpecificityThe mouse monoclonal antibody HC10 recognizes HLA class I heavy chains. HC10 reacts mostly with HLA-B and HLA-C heavy chains and some HLA-A (HLA-A10, HLA-A28, HLA-A29, HLA-A30, HLA-A31, HLA-A32, HLA-A33).
- Application Supplier NoteHC10 was raised against free class I heavy chains of HLA antigens to obtain antibodies that would still react with denatured class I antigens, as they occur in Western blotting, conventional light microscopical analysis of formalin-fixed, paraffin-embedded sections, and cryo-immuno-electron microscopy. HC10 indeed retains strong reactivity with free class I heavy chains in Western blots. HC10 also produces strong reactivity in immuno-electron microscopy. Its use allows the determination of tissue and subcellular distribution of class I antigen and can be used to specifically block free heavy chains (FHC); See reference 6. Optimal antibody dilutions for the different applications should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
- Applications SupplierELISA;Flow Cytometry;Immunohistochemistry (frozen & paraffin);Immunocytochemistry;Immunoprecipitation;Western Blotting;Immuno-cryo-electron microscopy;Immunohistochemistry (frozen & paraffin);ELISA;Immuno-cryo-electron microscopy
- Category SupplierPrimary antibodies
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHC10
- HostMouse
- IsotypeIgG2a
- SourceHC10 is a mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with HLA-B7 and -B40 heavy chains.
- ReactivityHuman
- Reactivity SupplierHuman
- UNSPSC12352203
References
- 1. Stam NJ, Vroom TM, Peters PJ, Pastoors EB, Ploegh HL. HLA-A- and HLA-B-specific monoclonal antibodies reactive with free heavy chains in western blots, in formalin-fixed, paraffin-embedded tissue sections and in cryo-immuno-electron microscopy. Int. Immunol. 1990;2:113-125. 2. de Kruijf EM, van Nes JGH, Sajet A, Tummers QRJG, Putter H, Osanto S, Speetjens FM, Smit VTHBM, Liefers GJ, van de Velde CJH, Kuppen PJK. The predictive value of HLA Class I tumor cell expression and presence of intratumoral Tregs for chemotherapy in patients with early breast cancer. Clin Cancer Res 2010;16:1272-1280. 3. Gauster M, Blashitz A, Dohr G. Monoclonal antibody HC10 does not bind to HLA-G. Rheumatology 2007; 46: 892-893. 4. Perosa F, Luccarelli G, Prete M, Favoino E, Ferrone S, Dammacco F. Beta-2-Microglobulin-free HLA Class I heavy chain epitope mimicry by monoclonal antibody HC-10-specific peptide. J Immunol 2003;171:1918-1926. 5. Hutter H, Hammer A, Blaschitz A, Hartmann M, Ebbesen P, Dohr G, Ziegler A, Uchanska-Ziegler B. Expression of HLA class I molecules in human first trimester and term placenta trophoblast. Cell Tissue Res 1996; 286:439–447. 6. Abdullah H, Zhang Z, Yee K, Haroon N. KIR3DL1 interaction with HLA-B27 is altered by ankylosing spondylitis associated ERAP1 and enhanced by MHC class I cross-linking. Discov Med. 2015;20(108):79-89 7. Goodridge JP, Burian A, Lee N, Geraghty DE. HLA-F and MHC class I open conformers are ligands for NK cell Ig-like receptors.J Immunol. 2013;191(7):3553-62