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Anti-HMHA1 Antibody

101-10724
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetARHGAP45
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Mouse Anti-Human HMHA1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID23526
  • Target name
    ARHGAP45
  • Target description
    Rho GTPase activating protein 45
  • Target synonyms
    HA-1; histocompatibility (minor) HA-1; HLA-HA1; HMHA1; minor histocompatibility antigen HA-1; minor histocompatibility protein HA-1; rho GTPase-activating protein 45
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    Human HMHA1 monoclonal antibody (100 ug)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ARHGAP45 using anti-ARHGAP45 antibody (A31863-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGAP45 antigen affinity purified polyclonal antibody (Catalog # A31863-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGAP45 at approximately 160 kDa. The expected band size for ARHGAP45 is at 125 kDa.
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