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ProductsBack/Mouse anti Human Keratin (4,5,6,8,10,13,18)

Mouse anti Human Keratin (4,5,6,8,10,13,18)

X1260M
Nordic-MUbio
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKRT5
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human Keratin (4,5,6,8,10,13,18)
  • Delivery Days Customer
    7
  • Application Supplier Note
    X1260M detects keratins 4,5,6,8,10,13 & 18 by Western blot. Immunohistochemistry can be performed on frozen tissues and paraffin sections. Optimal concentration should be evaluated by serial dilutions. X1260M reacts with a variety of normal and neoplastic epithelia. Reacting with simple epithelium and both basal and superbasal layers of cornifying and non-cornifying squamous epithelium this antibody is also useful in staining cultured epithelial cell lines. It is useful in differentiating epithelial tumors from non-epithelial tumors.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Immunohistochemistry (frozen & paraffin);Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C11
  • Conjugate
    Unconjugated
  • Gene ID3852
  • Target name
    KRT5
  • Target description
    keratin 5
  • Target synonyms
    58 kda cytokeratin; CK5; CK-5; cytokeratin-5; DDD; DDD1; EBS2; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin, type II cytoskeletal 5; KRT5A; type-II keratin Kb5
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP13647
  • Protein Name
    Keratin, type II cytoskeletal 5
  • Shelf life instruction
    See expiration date on vial
  • Source
    Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation from human A431 carcinoma cells and mouse myeloma cells.
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (A00398). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 5 antigen affinity purified polyclonal antibody (Catalog # A00398) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 5 at approximately 62 kDa. The expected band size for Cytokeratin 5 is at 62 kDa.
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