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Anti-LAMA2 Antibody

101-10891
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetLAMA2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Mouse Anti-Human LAMA2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    0.5 mg/ml
  • Gene ID3908
  • Target name
    LAMA2
  • Target description
    laminin subunit alpha 2
  • Target synonyms
    laminin M chain; laminin subunit alpha-2; laminin, alpha 2; laminin-12 subunit alpha; laminin-2 subunit alpha; laminin-4 subunit alpha; LAMM; MDC1A; merosin heavy chain; mutant laminin subunit alpha 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Human LAMA2 monoclonal antibody (100 ug)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Laminin using anti-Laminin antibody (A03522-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HCCT tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Laminin antigen affinity purified polyclonal antibody (Catalog # A03522-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Laminin at approximately 200 kDa. The expected band size for Laminin is at 177-200 kDa.
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