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Anti-MESP2 Antibody

101-10988
RayBiotech
ApplicationsELISA
Product group Antibodies
TargetMESP2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Mouse Anti-Human MESP2
  • Delivery Days Customer
    4
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID145873
  • Target name
    MESP2
  • Target description
    mesoderm posterior bHLH transcription factor 2
  • Target synonyms
    bHLHc6; class C basic helix-loop-helix protein 6; mesoderm posterior 2 homolog; mesoderm posterior basic helix-loop-helix transcription factor 2; mesoderm posterior protein 2; SCDO2
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    Human MESP2 monoclonal antibody (100 ug)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Mesp2 using anti-Mesp2 antibody (A07046-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mesp2 antigen affinity purified polyclonal antibody (Catalog # A07046-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mesp2 at approximately 45KD. The expected band size for Mesp2 is at 45KD.
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ELISA analysis of antigen using GTX60671 MESP2 antibody [1B3F9]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
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