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Anti-MMP28 Antibody

101-11010
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetMMP28
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Mouse Anti-Human MMP28
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID79148
  • Target name
    MMP28
  • Target description
    matrix metallopeptidase 28
  • Target synonyms
    EPILYSIN; matrix metalloprotease MMP25; matrix metalloproteinase-28; MM28; MMP25; MMP-25; MMP-28
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    Human MMP28 monoclonal antibody (100 ug)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MMP28 using anti-MMP28 antibody (A07364-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP28 antigen affinity purified polyclonal antibody (Catalog # A07364-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP28 at approximately 48KD. The expected band size for MMP28 is at 48KD.
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