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ProductsBack/Mouse anti Human p19 - ink4d

Mouse anti Human p19 - ink4d

C165M
Nordic-MUbio
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCDKN2D
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human p19 - ink4d
  • Delivery Days Customer
    7
  • Application Supplier Note
    Applications: Western Blot, Immunoistochemistry, Immunoprecipitation (co-precipitates cdk4/cdk6)
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    Western Blotting;Immunoprecipitation;Immunohistochemistry;Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DCS-100
  • Conjugate
    Unconjugated
  • Gene ID1032
  • Target name
    CDKN2D
  • Target description
    cyclin dependent kinase inhibitor 2D
  • Target synonyms
    INK4D, p19, p19-INK4D, cyclin-dependent kinase 4 inhibitor D, CDK inhibitor p19INK4d, cell cycle inhibitor, Nur77 associating protein, cyclin-dependent kinase 4 inhibitor D p19, cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4), inhibitor of cyclin-dependent kinase 4d
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP55273
  • Protein Name
    Cyclin-dependent kinase 4 inhibitor D
  • Scientific Description
    hp19; p19 ink4d; CDKN2D; Cyclin-dependent kinase 4 inhibitor D
  • Shelf life instruction
    See expiration date on vial
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    A his-tagged recombinant full length p19 protein recognizing a 19kD protein
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of P19 INK4d/CDKN2D using anti-P19 INK4d/CDKN2D antibody (A04390-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT4 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P19 INK4d/CDKN2D antigen affinity purified polyclonal antibody (Catalog # A04390-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P19 INK4d/CDKN2D at approximately 18 kDa. The expected band size for P19 INK4d/CDKN2D is at 18 kDa.
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