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ProductsBack/Mouse anti Human secretory component (free and bound), conjugated with Biotin

Mouse anti Human secretory component (free and bound), conjugated with Biotin

MAHu/SC/Bio
Nordic-MUbio
ApplicationsDot Blot, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetPIGR
0.2 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human secretory component (free and bound), conjugated with Biotin
  • Delivery Days Customer
    7
  • Application Supplier Note
    ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
  • Applications
    Dot Blot, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    Dot blot;ELISA;Immunoblotting;Immunocytochemistry;Immunohistochemistry
  • Category Supplier
    Secondary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    NI 194-4 (A89-39)
  • Conjugate
    Biotin
  • Gene ID5284
  • Target name
    PIGR
  • Target description
    polymeric immunoglobulin receptor
  • Target synonyms
    hepatocellular carcinoma associated protein TB6; poly-Ig receptor; polymeric immunoglobulin receptor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP01833
  • Protein Name
    Polymeric immunoglobulin receptor
  • Source
    Highly purified secretory component isolated from human milk.
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PIGR using anti-PIGR antibody (PB9776). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: SKOV Whole Cell Lysate, Lane 3: MCF-7 Whole Cell Lysate, Lane 4: SW620 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIGR antigen affinity purified polyclonal antibody (Catalog # PB9776) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIGR at approximately 83 kDa. The expected band size for PIGR is at 83 kDa.
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