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Mouse anti Human SMC-1

X1324M
Nordic-MUbio
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetSMC1A
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human SMC-1
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody can be used for Western blotting (1-5 microg/ml) and immunofluorescence (5-10 microg/ml). Optimal concentration should be evaluated by serial dilutions.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    Western Blotting;Immunofluorescence
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C2M
  • Conjugate
    Unconjugated
  • Gene ID8243
  • Target name
    SMC1A
  • Target description
    structural maintenance of chromosomes 1A
  • Target synonyms
    CDLS2, DEE85, DXS423E, EIEE85, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB, structural maintenance of chromosomes protein 1A, SMC protein 1A, SMC-1-alpha, SMC1 (structural maintenance of chromosomes 1, yeast)-like 1, epididymis secretory sperm binding protein, segregation of mitotic chromosomes 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ14683
  • Protein Name
    Structural maintenance of chromosomes protein 1A
  • Shelf life instruction
    See expiration date on vial
  • Source
    Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant protein corresponding to amino acids 402-894 of human SMC1 and mouse myeloma cells.
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SMC1A using anti-SMC1A antibody (A02148-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC1A antigen affinity purified polyclonal antibody (Catalog # A02148-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC1A at approximately 150 kDa. The expected band size for SMC1A is at 143 kDa.
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