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Anti-TBX18 Antibody

101-11743
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetTBX18
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-TBX18 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID9096
  • Target name
    TBX18
  • Target description
    T-box transcription factor 18
  • Target synonyms
    CAKUT2, PUJO, T-box transcription factor TBX18, T-box 18, T-box protein 18
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    Human TBX18 monoclonal antibody (100 ug)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TBX18 using anti-TBX18 antibody (A06044-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBX18 antigen affinity purified polyclonal antibody (Catalog # A06044-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBX18 at approximately 70 kDa. The expected band size for TBX18 is at 70 kDa.
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