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ProductsBack/Mouse anti Integrin alpha 6B

Mouse anti Integrin alpha 6B

MUB0905P
Nordic-MUbio
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetITGA6
0.1 mg
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Integrin alpha 6B
  • Delivery Days Customer
    7
  • Application Supplier Note
    6B4 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    Immunocytochemistry;Immunohistochemistry (frozen);Western Blotting
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6b4
  • Gene ID3655
  • Target name
    ITGA6
  • Target description
    integrin subunit alpha 6
  • Target synonyms
    CD49 antigen-like family member F; CD49f; integrin alpha-6; integrin alpha6B; integrin, alpha 6; ITGA6B; VLA-6
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP23229
  • Protein Name
    Integrin alpha-6
  • Source
    6B4 is a Mouse monoclonal IgG1, kappa antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a synthetic peptide corresponding to a 34 amino acid stretch in the cytoplasmic domain of integrin ...
  • Reactivity Supplier
    Human
  • UNSPSC
    12352203

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Lane 1: SW480 cell lysates; Lane 2: A549 cell lysates; Lane 3: Huvec cell lysates; Lane 4: A431 cell lysates probed with Integrin alpha 6 Polyclonal Antibody, Unconjugated (bs-2641R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of ITGA6 using anti-ITGA6 antibody (A01693). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA6 antigen affinity purified polyclonal antibody (Catalog # A01693) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA6 at approximately 127 kDa. The expected band size for ITGA6 is at 127 kDa.
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