Mouse anti Nesprin-3a

Nordic-MUbio

Mouse anti Nesprin-3a

7

Nsp3 reacts specifically with nesprin-3a.

Nsp3 is useful for immunohistochemistry on frozen tissues and cell cultures fixed with methanol, or 4% paraformaldehyde combined with a permeabilization step of 15 in 0.1% Triton in PBS. The antibody is also suited for immunoblotting. Optimal antibody dilution should be determined by titration. Recommended range is 1:100 - 1:1000 for immunohistochemistry with FITC-conjugated secondary antibodies or avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagents.

Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen

Immunohistochemistry (frozen);Immunoblotting;Immunocytochemistry

Primary antibodies

Research Use Only

Monoclonal

Nsp-3

Syne3

spectrin repeat containing, nuclear envelope family member 3

KASH domain-containing protein 3; KASH3; nes; nesprin-3; nuclear envelope spectrin repeat protein 3

Mouse

IgG

Nsp3 is a mouse monoclonal antibody made by immunization of Balb/c mice with the GST fusion protein encoding the seventh spectrin repeat of NSP-3a.

Mouse

Mouse

12352203