Mouse anti Plakophilin-3

Nordic-MUbio

Mouse anti Plakophilin-3

7

12B11F8 is suitable for immunocytochemistry (methanol-fixed cells), immunohistochemistry on frozen tissues, immunoprecipitation and Western blot detection. For frozen tissues use a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For optimal results, fixed cells or tissues should be treated with 0.2% Triton-X 100 for 15 min. prior to incubation with primary antibody. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen

Immunocytochemistry;Immunohistochemistry (frozen);Immunoprecipitation;Western Blotting

Research Use Only

Monoclonal

12B11F8

PKP3

plakophilin 3

plakophilin-3, plakophilin 3b

Mouse

IgG2a

Plakophilin-3

12B11F8 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a synthetic peptide corresponding to the extreme carboxyterminal amino acid residues 779-793 (KLHRDFRAKGYRKED) of ...

Canine;Human;Mouse;Swine

12352203