Denatured rabbit IgG (8 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Mouse anti-Rabbit IgG Light chain (GTX628142) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with DDDDK tag antibody [M2-RB] (GTX640415) (1 μg/mL). Mouse Anti-Rabbit IgG (Light chain) antibody [GT1779] (HRP) (GTX628142-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628142-01/GTX628142-01_44783_20240802_ELISA_Indirect_24080602_410.webp "Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (GTX130342-pro) (57.14-0.89 nM). Coated protein was probed with DDDDK tag antibody [M2-RB] (GTX640415) (1 μg/mL). Mouse Anti-Rabbit IgG (Light chain) antibody [GT1779] (HRP) (GTX628142-01) (1:10000) was used to detect the bound primary antibody.")
Denatured rabbit IgG (8 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Mouse anti-Rabbit IgG Light chain (GTX628142) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Mouse Anti-Rabbit IgG (Light chain) antibody [GT1779] (HRP)
GTX628142-01
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityRabbit
Overview
- SupplierGeneTex
- Product NameMouse Anti-Rabbit IgG (Light chain) antibody [GT1779] (HRP)
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1779
- Concentration1.27 mg/ml
- ConjugateHRP
- HostMouse
- IsotypeIgG2a
- ReactivityRabbit
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161