Bio-Connect

Mouse anti-SARS-CoV2

ORB1496480
Biorbyt
ReactivityMouse
Product group Assays
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Mouse anti-SARS-CoV2
  • Delivery Days Customer
    10
  • Application Supplier Note
    standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S-BRD IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S-BRD IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S-BRD IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mouse S-BRD IgG in the samples is then determined by comparing the OD of the samples to the standard curve
  • Assay Detection Range
    1.57-100 ng/mL
  • Assay Sensitivity
    0.91 ng/mL
  • Certification
    Research Use Only
  • Scientific Description
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S-BRD IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S-BRD IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S-BRD IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mouse S-BRD IgG in the samples is then determined by comparing the OD of the samples to the standard curve.
  • Reactivity
    Mouse
  • UNSPSC
    41116133