Mouse anti Smoothelin

Nordic-MUbio

Mouse anti Smoothelin

7

R4A reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells.

R4A is suitable for immunoblotting, immunocytochemistry on permeabilised cells, and and immunohistochemistry on frozen and paraffin-embedded tissues. For staining paraffin-embedded tissues pretreatment in 10 mM citRate buffer (pH 6.0) and heating for 3 times 5 minutes in a microwave is required. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Immunohistochemistry (frozen);Immunohistochemistry (paraffin);Western Blotting;Immunocytochemistry

Primary antibodies

Research Use Only

Monoclonal

R4A

SMTN

smoothelin

smoothelin

Mouse

IgG1

Smoothelin

R4A is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal extract of chicken gizzard.

Canine, Chicken, Feline, Human, Monkey, Porcine

Canine;Chicken;Feline;Human;Monkey;Swine

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