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ProductsBack/Mouse CD154/CD40L Antibody (OASB01689)

Mouse CD154/CD40L Antibody (OASB01689)

OASB01689
Aviva Systems Biology
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
Product group Antibodies
TargetCd40lg
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    Mouse CD154/CD40L Antibody (OASB01689)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MR1
  • Concentration
    0.5 mg/ml
  • Gene ID21947
  • Target name
    Cd40lg
  • Target description
    CD40 ligand
  • Target synonyms
    CD154; Cd40; CD40 ligand; Cd40l; CD40-L; gp3; gp39; HIGM; HIGM1; IGM; IMD; IMD3; Ly-6; Ly62; Ly-62; T-BA; T-BAM; T-cell antigen Gp39; TNF-related activation protein; Tnfs; Tnfsf5; Tnlg8b; TRAP; tumor necrosis factor ligand 8b; tumor necrosis factor ligand superfamily member 5
  • Host
    Hamster
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD40L using anti-CD40L antibody (A01114-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40L antigen affinity purified polyclonal antibody (Catalog # A01114-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40L at approximately 36KD. The expected band size for CD40L is at 29KD.
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FACS analysis of mouse C57Bl/6 T cells (enriched from total splenocytes, with PMA and Ionomycin stimulation for 6 hrs) using GTX01485-02 CD40L / CD154 antibody [MR1] (Biotin). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.25 microg (5 microl)
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