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ProductsBack/PE Anti-Mouse CD183/CXCR3 Antibody [CXCR3-173]

PE Anti-Mouse CD183/CXCR3 Antibody [CXCR3-173]

AGEL0430
Assay Genie
ApplicationsFlow Cytometry
Product group Antibodies
TargetCxcr3
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Mouse CD183 Monoclonal Antibody (PE Conjugated) [CXCR3-173] (AGEL0430)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Applications Supplier
    FCM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    RPE
  • Gene ID12766
  • Target name
    Cxcr3
  • Target description
    C-X-C motif chemokine receptor 3
  • Target synonyms
    Cd183, Cmkar3, C-X-C chemokine receptor type 3, IP-10 receptor, chemokine (C-X-C motif) receptor 3, interferon-inducible protein 10 receptor
  • Host
    Hamster
  • Protein IDO88410
  • Protein Name
    C-X-C chemokine receptor type 3
  • Shelf life instruction
    12 months
  • Source
    Armenian Hamster
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cxcr3 using anti-Cxcr3 antibody (A00295-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cxcr3 antigen affinity purified polyclonal antibody (Catalog # A00295-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cxcr3 at approximately 46 kDa. The expected band size for Cxcr3 is at 41 kDa.
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HCCLM3 cells probed with Rabbit Anti-CXCR3 Polyclonal Antibody, Unconjugated (bs-2209R ) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (bs-0295G-FITC) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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