Mouse COL6A1 protein, His tag

GeneTex

Mouse COL6A1 protein (active)

9

Collagen alpha-1(VI) chain (COL6a1) is a protein that in humans, the collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Besides, Platelet-derived growth factor subunit A (PDGFA) has been identified as an interactor of COL6A1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human COL6a1 and recombinant human PDGFA. Briefly, COL6a1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 microl were then transferred to PDGFA-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-COL6a1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 microl stop solution to the wells and read at 450nm immediately. The binding activity of COL6a1 and PDGFA was in a dose dependent manner.

Functional Assay

Research Use Only

Unconjugated

Collagen alpha-1(VI) chain

-20°C or -80°C,2°C to 8°C

12352202