Mouse COX1(Cytochrome C Oxidase Subunit I) ELISA Kit

Biorbyt

Mouse COX1(Cytochrome C Oxidase Subunit I) ELISA Kit

10

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse COX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse COX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse COX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mouse COX1 in the samples is then determined by comparing the OD of the samples to the standard curve

0.32-20 ng/mL

0.127 ng/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit I(COX1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome C Oxidase Subunit I(COX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C Oxidase Subunit I(COX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome C Oxidase Subunit I(COX1) in the samples is then determined by comparing the OD of the samples to the standard curve.

41116158