ELISA plate was coated with Goat Anti-Mouse IgG, Human ads-UNLB (SB Cat. No. 1030-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgA-BIOT (SB Cat. No. 0106-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.