Dot Blot showing the detection of Mouse IgG. A three-fold serial dilution of Mouse IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto (p/n B501-0500) 1 Hour at 20°C, Anti-Mouse IgG (H&L) (GOAT) Antibody Fluorescien Conjugated (p/n 610-102-121) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP.
Dot Blot showing the detection of Mouse IgG. A three-fold serial dilution of Mouse IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto (p/n B501-0500) 1 Hour at 20°C, Anti-Mouse IgG (H&L) (GOAT) Antibody Fluorescien Conjugated (p/n 610-102-121) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP.
Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed
TA397821
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
Overview
- SupplierOriGene
- Product NameMouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed
- Delivery Days Customer14
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- HostGoat
- Scientific DescriptionMouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed
- ReactivityMouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203