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ProductsBack/Mouse IgG1 Negative Control

Mouse IgG1 Negative Control

Research Use Only
0G11
Nordic-MUbio
ApplicationsFlow Cytometry
Product group Antibodies
TargetIl4
Price on request
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse IgG1 Negative Control
  • Delivery Days Customer
    7
  • Application Supplier Note
    Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood. PBMC: Add 10 microl of MAB/10^6 PBMC in100 microl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 microl of MAB/100 microl of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at ?633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flow Cytometry
  • Category Supplier
    Control antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ZX3
  • Conjugate
    Unconjugated
  • Gene ID16189
  • Target name
    Il4
  • Target description
    interleukin 4
  • Target synonyms
    B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; IGG1 induction factor; Il; Il-4; interleukin-4; lymphocyte stimulatory factor 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP07750
  • Protein Name
    Interleukin-4
  • Scientific Description
    IgG1
  • Shelf life instruction
    See expiration date on vial
  • UNSPSC
    12352203

References

  • 1. Immunofluorescence Measurement in a Flow Cytometer using Low-Power Helium Neon Laser Excitation. Shapiro, H.M, Glazer, A.N., Christenson, L., Williams, J.M., and Strom, T. B. Cytometry 4,276, 1983. 2. Comparison of Helium Neon and Dye lasers for Excitation of Allophycocyanin. Loken, M.R., Kiej, J.F. and Kelly, K.,A. Cytometry 8, 96, 1987. Cy-5 + Portions of this product is manufactured under license from Carnegie Mellon University, U.S. Patent Number 5,268,486.