Mouse IgG2a Isotype Control antibody [PPV-04]

Arigo Biolaboratories

Mouse IgG2a Isotype Control antibody [PPV-04]

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This isotppe control antibody is intended as Mouse IgG2b isotype control for the analysis to establish the amount of non-specific antibody binding. Using the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. In addition, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution using in the experiment. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 microg per ml of incubation mixture), change the conditions of your experiment to reduce the background. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Flow Cytometry, Other Application

Research Use Only

Monoclonal

PPV-04

1 mg/ml

Unconjugated

>95%

Mouse

IgG2a

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 microg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-04, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

-20°C

41116161