ELISA plate was coated with Goat Anti-Mouse IgG1, Human ads-UNLB (SB Cat. No. 1070-01), Goat Anti-Mouse IgG2c, Human ads-UNLB (SB Cat. No. 1079-01), Goat Anti-Mouse IgG2b, Human ads-UNLB (SB Cat. No. 1090-01), Goat Anti-Mouse IgG3, Human ads-UNLB (SB Cat. No. 1100-01), Goat Anti-Mouse IgA-UNLB (SB Cat. No. 1040-01), and Goat Anti-Mouse IgM, Human ads-UNLB (SB Cat. No. 1020-01). Serially diluted Mouse IgG2c-BIOT (SB Cat. No. 0122-08) was captured followed by Streptavidin-HRP (SB Cat. No. 7100-05) and quantified.