ELISA Results of Goat Anti-Mouse IgG3 (Gamma 3 chain) Antibody Minimum Cross Reactivity to Bovine, Human, and Rabbit Serum Proteins tested against purified Mouse IgG3 mx3. Each well was coated in duplicate with 1.0 µg of Mouse IgG3 (p/n 010-0143). The working dilution is 1:90,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).