Mouse IL-27 (Interleukin 27) ELISA (Small Sample Volume)

Assay Genie

Mouse IL-27 (Interleukin 27) ELISA (Small Sample Volume)

12

ELISA

31.25-2000 pg/mL

19.12 pg/mL

Research Use Only

Interleukin-27 subunit alpha

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL-27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL-27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mouse IL-27 in the samples is then determined by comparing the OD of the samples to the standard curve.

2°C to 8°C

41116100

Mouse