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ProductsBack/Mouse IL-3 Antibody : Biotin (OASB01266)

Mouse IL-3 Antibody : Biotin (OASB01266)

OASB01266
Aviva Systems Biology
ApplicationsWestern Blot, ELISpot Assay, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
Product group Antibodies
TargetIl3
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    Mouse IL-3 Antibody : Biotin (OASB01266)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISpot Assay, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MP2-43D11
  • Concentration
    0.5 mg/ml
  • Conjugate
    Biotin
  • Gene ID16187
  • Target name
    Il3
  • Target description
    interleukin 3
  • Target synonyms
    BPA, Csfmu, HCGF, Il-3, MCGF, PSF, interleukin-3, P-cell-stimulating factor, hematopoietic growth factor, mast cell growth factor, multipotential colony-stimulating factor
  • Host
    Rat
  • Isotype
    IgG2a
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL3 using anti-IL3 antibody (A02435). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL3 antigen affinity purified polyclonal antibody (Catalog # A02435) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL3 at approximately 26KD. The expected band size for IL3 is at 26KD.
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