HEK293T cells were transfected with pCMV6-Entry-AsRed2 cDNA for 48 hours. Serial dilution of AsRed2 expression lysates were separated by SDS-PAGE and immunoblotted with anti-AsRed2 (TA180099, 1:1000) (Lane 1, 5ug; Lane 2, 2.5ug; Lane 3, 1.25ug; Lane 4, 0.625ug; Lane 5, 0.3125ug).