Anti-Hu CD326 Purified

EXBIO

Anti-Hu CD326 Purified

7

Western blotting: Non-reducing conditions; recommended dilution: 1-2 microg/ml; positive control: MCF-7 cells. Immunoprecipitation: Recommended dilution: 1-4 microg / ml per 100-500 microg of protein in 1 ml lysate. Immunohistochemistry (paraffin sections): Recommended dilution: 1-10 microg/ml; pretreatment: tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0); positive tissue: breast carcinoma. Immunocytochemistry: Recommended dilution: 1-10 microg/ml. Flow cytometry: Recommended dilution: 1-4 microg/ml.

Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

323/A3

1 mg/ml

IgG1

CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.

Human, Rat

2°C to 8°C

12352203