Anti-GAPDHS Purified

EXBIO

Anti-GAPDHS Purified

7

Immunocytochemistry: Recommended dilution: 10 microg/ml; membrane permeabilization (acetone) is essential. Flow cytometry: Recommended dilution: 3-12 microg/ml. Intraacrosomal staining.

Flow Cytometry, Western Blot, ImmunoCytoChemistry

Research Use Only

Hs-8

1 mg/ml

IgM

GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Human, Porcine

2°C to 8°C

12352203