Anti-LIME1 Purified

EXBIO

Anti-LIME1 Purified

7

Immunohistochemistry (paraffin sections): Recommended dilution: 10 microg/ml. Western blotting: Reducing conditions; recommended dilution: 1 microg/ml. Flow cytometry: Recommended dilution: 1-5 microg/ml.

Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

LIME-10

1 mg/ml

IgG2a

LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

Human

2°C to 8°C

12352203