Mouse PDGFA protein, His tag (active)

GeneTex

Mouse PDGFA protein (active)

9

PDGFA (Platelet-derived growth factor subunit A) is a Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA has been described as a chemoattractant for monocytes and proven to be able to induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of PDGFA on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100 microl cell suspension, 1x106cells/ml in RPMI-1640 with 0. 5% FBS) and PDGFA (1. 5 ng/ml, 7. 5 ng/ml and 15 ng/ml diluted separately in serum free RPMI-1640 ) was added in lower chamber with a polycarbonate filter (8microm pore size) used to separate the two compartments. After incubation at 37C with 5% CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x40) and the number of migrated cells were counted at high magnification (x400) randomly. PDGFA is able to induce migration of THP-1 cells, and the optimum chemotaxis of PDGFA occurs at 1. 5 ng/ml.

Functional Assay

Research Use Only

Unconjugated

Platelet-derived growth factor subunit A

-20°C or -80°C,2°C to 8°C

12352202