Mouse SCF protein, His tag (active)

GeneTex

Mouse SCF protein, His tag (active)

9

Stem cell factor (also known as SCF, KIT-ligand, KL, or steel factor) is a cytokine that binds to the c-KIT receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. SCF has been shown to stimulate the proliferation of Raw264. 7 cells. To test this effect, Raw264. 7 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach later, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM containing various concentrations of recombinant mouse SCF. After incubated for 120h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 microl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37C. Proliferation of Raw264. 7 cells after incubation with SCF for 120h observed by inverted microscope.

Functional Assay

Research Use Only

Unconjugated

Kitl

kit ligand

Clo, Con, Gb, Kitlg, Mgf, SCF, SF, SLF, Sl, blz, contrasted, kit ligand, C-kit ligand, Steel factor, cloud gray, grizzle-belly, hematopoietic growth factor KL, mast cell growth factor, stem cell factor

Kit ligand

-20°C or -80°C,2°C to 8°C

41116120

Mouse